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To measure cytochrome P450 activity, on the day of the experiment cholangiocyte and hepatocyte organoids in EM2 or DM were removed from Matrigel using Cell Recovery solution (Corning)

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Confocal images are acquired with 405 nm laser/418495 nm filter and 488 nm laser/499615 nm filter

Both in vivo and in vitro, acetaminophen can induce apoptosis by triggering the release of mitochondrial cytochrome-c and activating the apoptotic pathway 48,49